Electron Microscopy
Our Electron Microscopy Core provides innovative ultrastructural visualization technologies based on collaborations with investigators, offering expertise, training, and support in the latest technologies and advances in the field.
Overview
The Electron Microscopy Core Facility provides technical support for electron microscopy (EM) sample preparation, training in the use of electron microscopes and other technologies in the lab, and collaborates with researchers in the design of EM experiments and data interpretation. The Electron Microscopy Core develops novel techniques alongside investigators to overcome limitations in research projects, providing services to scientists at MPFI and other research institutions.
Our Services
The following applications are being offered:
- Correlative light and electron microscopy (CLEM)
- Conventional ultrathin-section TEM to visualize pure morphology with high-resolution
- Thin-section based pre- and post- embedding immuno-EM to localize target molecules of interest
- Freeze-fracture replica immunogold labeling to localize target membrane proteins, including all types of receptors and channels in membrane structures two-dimensionally
- Negative staining to visualize small molecules and others
- Serial Block Face SEM for 3D-reconstruction of large volumes of data
- Thin-section based array tomography with serial-SEM imaging
- Conventional SEM
Browse Our Equipment
Gemini SEM300 equipped with Gatan 3view
Merlin VP Compact Scanning Electron Microscope
HPM100 High Pressure Freezing
Automated Tape-Collecting Ultramicrotome (ATUM)
Glow Discharger (PELCO easiGlow)
Tecnai Biotwin Transmission Electron Microscope
ACE600 Sputter and eBeam Coating
Automatic Freeze Substitution
Freeze Fracture System
Cryo-Plunger System
Contact
Debbie Guerrero-Given
Manager, Electron Microscopy Core
emc@mpfi.org