Light Microscopy Core provides state-of-the-art technology for imaging living cells and tissues and collaborates with investigators to develop and implement novel imaging technologies.

Access to technology and expertise in fluorescence imaging is a critical need in advanced scientific research. Current fluorescence imaging instruments are expensive, complicated, and require continued maintenance and improvements. The complexity of the technology is often difficult to understand and hard for individual laboratories to adapt, especially when optical imaging is not their major focus. The facility meets this need for both investigators at MPFI, as well as those in the neighboring scientific community. Additionally, the Optical Workshop provides on-site services for individual laboratories such as consultation, installation, troubleshooting, modification, and training. The facility participates actively in MPFI’s high-school and undergraduate internship programs.

Our Services

  • High sensitivity point scanning confocal imaging
  • Parallel multi-color imaging
  • Multi-point time-lapse imaging
  • Ultra-fast resonant scanning for reduced bleaching and photo-damage (particularly for live-cell imaging)
  • Full microscope enclosure for live-cell imaging High-throughput generation of large 3D data stacks for use in 3D reconstructions
  • Improved signal-to-noise (SNR) and spatial resolution based on image reconstruction using the Airyscan detection system
  • Various techniques for studying molecular dynamics and interactions, such as FRET, Anisotropy, FRAP, FLIP and FCS

Equipment

Zeiss LSM 880 with Airyscan

(inverted stage; 7 excitation lasers @405nm, 458nm, 488nm, 514nm, 561nm, 594nm & 633nm; confocal detection with high-sensitivity GaAsP detector & 2 PMTs, spectrally configurable between 390nm – 750nm; Airyscan module; motorized stage; automatic focus-correction; objectives: 10x/0.45 air, 20x/0.80 air, 40x/1.2 water, 63x/1.4 oil)

Zeiss LSM 780

(upright stage; 7 excitation lasers @405nm, 458nm, 488nm, 514nm, 561nm, 594nm & 633nm; confocal detection with high-sensitivity GaAsP detector & 2 PMTs, spectrally configurable between 390nm – 750nm; photon-counting; objectives: 10x/0.40 air, 20x/0.80 air, 25x/0.8 multi-corr, 40x/1.3 Oil and 63x/1.3 multi-corr)

Leica TCS SP5 II with resonant scanner

(upright stage; 6 excitation lasers @405nm, 458nm, 488nm, 514nm, 561nm & 633nm; 8kHz resonant-scanner; confocal detection: 3 hybrid- & two regular PMTs, spectrally configurable; motorized scanning stage; objectives: 10x/0.40 air, 20x/0.70 air, 40x/1.25 oil, 63x/1.40 oil and 63x /0.9 water)

Contact

Long Yan
Head of Light Microscopy
(561) 972-9043
long.yan@mpfi.org